Isotonic Solution:
Solution having osmolarity same as that of plasma is known as an
Isotonic Solution.
Eg: 0.9% NaCl, 5% Glucose, 10% Urea, 20% Mannitol
RBCs when put into isotonic solution, no change in size takes place.
Hypotonic Solution:
Solution having osmolarity less than that of plasma is known as
Hypotonic Solution.
Eg: 0.6% NaCl
RBCs when put into hypotonic solution, endosmosis takes place,
that is RBCs enlarge & finally bursts. A burst cell is known as ‘Ghost
Cell’.
Hypertonic Solution:
Solution having osmolarity greater than that of plasma is known as
Hypertonic Solution.
Eg:
RBCs when put into hypertonic solution, exosmosis takes place,
that is RBCs shrunk and become Creanted RBCs. This phenomenon is known as ‘Crenation’.
Picture Source: chemistry.about.com
Apparatus Required:
Microscope
Equipments for aseptic procedure – wet/spirit swab (70%f
alcohol dipped swab)
3 glass slides + 3 cover slips + 3 tooth pics